flow cytometry: a novel approach for indirect assessment of protamine deficiency by cma3 staining, taking into account the presence of m540 or apoptotic bodies

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Flow Cytometry: A Novel Approach for Indirect Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies

BACKGROUND Chromomycin A3 (CMA3) staining, either by the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool to improve assessment accuracy, both in terms of statistical analysis and for prevention of observer variation. This study provides a simple procedure to account for merocyanin...

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P-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies

Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...

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Flow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency

Background Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. FCM provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. During spermiogenesis histones are replaced by prota...

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flow cytometry: a new approach for indirect assessment of sperm protamine deficiency

background: flow cytometry (fcm) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. fcm provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. during spermiogenesis histones are replaced by prot...

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Journal title:
international journal of fertility and sterility

جلد ۵، شماره ۳، صفحات ۱۲۸-۱۳۳

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